Anticorps Polyclonal de lapin anti-EIF2C3-Specific

EIF2C3-Specific Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 19692-1-AP

Synonymes

AGO3, Argonaute 3, Argonaute3, eIF 2C 3, eIF2C 3, EIF2C3, hAgo3, Protein argonaute 3

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Galerie de données de validation

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  WB analysis of SKOV-3 using 19692-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

19692-1-AP cible EIF2C3-Specific dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2C, 3
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_024852
• Symbole du gène: EIF2C3
• Identification du gène (NCBI): 192669
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2C3, also named as AGO3, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi). EIF2C3 binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. EIF2C3 lacks endonuclease activity and does not appear to cleave target mRNAs. This antibody is specific to EIF2C3.

Publications

Species	Application	Title
human	WB	Nat Struct Mol Biol; A novel class of microRNA-recognition elements that function only within open reading frames.; Authors - Kai Zhang