Anticorps Polyclonal de lapin anti-EIF3E

EIF3E Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10899-1-AP

Synonymes

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

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Galerie de données de validation

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  WB analysis of rat lymph using 10899-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10899-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10899-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10899-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10899-1-AP

  IP result of anti-EIF3E (IP:10899-1-AP, 4ug; Detection:10899-1-AP 1:500) with Jurkat cells lysate 2440 ug.

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  IHC staining of human pancreas cancer using 10899-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10899-1-AP (EIF3E antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of U2OS using 10899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 10899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu lymphatique de rat, cellules HeLa, cellules Jurkat, tissu de thymus de souris, tissu pancréatique de souris
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10899-1-AP cible EIF3E dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF3E Protéine recombinante Ag1132
• Nom complet: eukaryotic translation initiation factor 3, subunit E
• Masse moléculaire calculée: 445 aa, 52 kDa
• Poids moléculaire observé: 48-52 kDa
• Numéro d’acquisition GenBank: BC008419
• Symbole du gène: EIF3E
• Identification du gène (NCBI): 3646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Aging Cell; Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells.; Authors - Yang You
human	IHC	Cancer Genomics Proteomics; Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate; Authors - MOHAMED E. M. SAEED
human	WB	Cancer Lett; N6-Methyladenosine enhances the translation of ENO1 to promote the progression of bladder cancer by inhibiting PCNA ubiquitination; Authors - Chengquan Shen
pig	WB	J Virol; VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1; Authors - Lu Li

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