Anticorps Polyclonal de lapin anti-EIF4E3

• Phare
• Validé par KD/KO

EIF4E3 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 17282-1-AP

Synonymes

eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3

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Galerie de données de validation

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  WB analysis of HeLa using 17282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17282-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17282-1-AP

  Immunofluorescent analysis of Hela cells, using EIF4E3 antibody 17282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, tissu cardiaque de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17282-1-AP cible EIF4E3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF4E3 Protéine recombinante Ag11056
• Nom complet: eukaryotic translation initiation factor 4E family member 3
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24-30 kDa
• Numéro d’acquisition GenBank: BC031289
• Symbole du gène: EIF4E3
• Identification du gène (NCBI): 317649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918). EIF4E3 exists two isoforms with MV 24 kDa and 13 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 30382096; Authors - Nourhan Abdelfattah ; KD Validated
human	WB, IP	Nat Commun; MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA translation in DLBCL.; Authors - Ari L Landon
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Mol Ther; RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.; Authors - Jiexin Li
human	WB	iScience; Transcriptome, proteome, and protein synthesis within the intracellular cytomatrix; Authors - Tattym E Shaiken
human	WB	Oncotarget; Noncanonical SQSTM1/p62-Nrf2 pathway activation mediates proteasome inhibitor resistance in multiple myeloma cells via redox, metabolic and translational reprogramming.; Authors - Irene Riz ; KD Validated