Anticorps Polyclonal de lapin anti-ELAC2

• Phare
• Validé par KD/KO

ELAC2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 10071-1-AP

Synonymes

elaC homolog 2 (E. coli), ElaC homolog protein 2, ELAC2, ELC2, HPC2, Ribonuclease Z 2, RNase Z 2, tRNA 3 endonuclease 2, tRNase Z 2

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Galerie de données de validation

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  WB analysis of DU 145 using 10071-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10071-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10071-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 10071-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10071-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Informations sur le produit

10071-1-AP cible ELAC2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELAC2 Protéine recombinante Ag0105
• Nom complet: elaC homolog 2 (E. coli)
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC004158
• Symbole du gène: ELAC2
• Identification du gène (NCBI): 60528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	IF	Cell Metab; GRSF1 regulates RNA processing in mitochondrial RNA granules.; Authors - Jourdain Alexis A AA
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen
human	WB	Nat Commun; Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria.; Authors - Zbigniew Pietras
mouse	WB	Cell Rep; Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.; Authors - Oliver Rackham
human	WB,IF	RNA Biol; Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs.; Authors - Brzezniak Lien K LK ; KD Validated

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