Anticorps Monoclonal anti-ELAVL2

ELAVL2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2D8C10

Avis (1)

N° de cat : 67097-1-Ig

Synonymes

ELAV like neuronal protein 1, ELAV like protein 2, ELAV2, ELAVL2, HEL N1, HELN1, Hu antigen B, HUB

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Galerie de données de validation

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  WB analysis using 67097-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of Neuro-2a using 67097-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67097-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67097-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67097-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 67097-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67097-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67097-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 67097-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67097-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Neuro-2a, cellules PC-12, cellules SH-SY5Y, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67097-1-Ig cible ELAVL2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ELAVL2 Protéine recombinante Ag28310
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC030692
• Symbole du gène: ELAVL2
• Identification du gène (NCBI): 1993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Publications

Species	Application	Title
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
mouse	WB	Sci Adv; The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity; Authors - Dominika Grzejda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Petr (Verified Customer) (07-19-2023)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000