Anticorps Polyclonal de lapin anti-HuA/B/C/D

HuA/B/C/D Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13032-1-AP

Synonymes

ELAV like protein 4, ELAVL4, Hu antigen D, Hu C/D, HuA/B/C/D, HUD, PNEM

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Galerie de données de validation

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  WB analysis of human cerebellum using 13032-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 13032-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13032-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 13032-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13032-1-AP

  IP Result of anti-HuA/B/C/D (IP:13032-1-AP, 4ug; Detection:13032-1-AP 1:300) with mouse brain tissue lysate 2560ug.

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  IHC staining of human brain using 13032-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13032-1-AP (HuA/B/C/D antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 13032-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13032-1-AP (HuA/B/C/D antibody at dilution of 1:200 (under 10x lens).

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  IF Staining of mouse brain using 13032-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuA/B/C/D antibody (13032-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de cervelet humain, cellules PC-13, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

13032-1-AP cible HuA/B/C/D dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HuA/B/C/D Protéine recombinante Ag3678
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Masse moléculaire calculée: 380 aa, 42 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC036071
• Symbole du gène: ELAVL4
• Identification du gène (NCBI): 1996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].

Publications

Species	Application	Title
rat	IHC	J Neurosci Methods; Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF.; Authors - Ruth Butler-Ryan
human	IHC,WB	J Cosmet Dermatol; Study on the correlation of high expression level of HuD with seborrheic keratosis and skin aging; Authors - Juping Chen
mouse	IF	Med Mol Morphol; Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T mice; Authors - Nami O Yamada
mouse	IF	Biomed Pharmacother; Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axis; Authors - Jinzhao Bai