Anticorps Polyclonal de lapin anti-ELK1

• Phare
• Validé par KD/KO

ELK1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 27420-1-AP

Synonymes

ELK , ELK1

---

Galerie de données de validation

• 

  WB analysis using 27420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27420-1-AP

  WB result of ELK1 antibody (27420-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELK1 transfected HepG2 cells.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

27420-1-AP cible ELK1 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELK1 Protéine recombinante Ag26683
• Nom complet: ELK1, member of ETS oncogene family
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC056150
• Symbole du gène: ELK1
• Identification du gène (NCBI): 2002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	WB	Front Oncol; tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway; Authors - Weiguo Xu
rat	IHC	J Transl Med; The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy.; Authors - Xue Li
human	WB	Biochem Biophys Res Commun; CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p.; Authors - Ranran Sun
human	WB	Genes Genomics; Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.; Authors - Akhlaq Ahmad ; KD Validated
human	WB	Cancer Res Commun; Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer; Authors - Liwei Lang ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}