Anticorps Polyclonal de lapin anti-ELK4

ELK4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ChIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 14666-1-AP

Synonymes

ELK4, SAP 1, SAP1, SRF accessory protein 1

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Galerie de données de validation

• 

  WB analysis of HeLa using 14666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14666-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14666-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• ChIP: See 1 publications below

Informations sur le produit

14666-1-AP cible ELK4 dans les applications de WB, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELK4 Protéine recombinante Ag6273
• Nom complet: ELK4, ETS-domain protein (SRF accessory protein 1)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC063676
• Symbole du gène: ELK4
• Identification du gène (NCBI): 2005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELK4, also named as SAP1, SAP-1 and SAP1, belongs to the ETS family. It forms a ternary complex with the serum response factor (SRF). ELK4 requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. It is androgen regulated, involved in promoting cell growth, and highly expressed in a subset of prostate cancer. (PMID:19293179 )

Publications

Species	Application	Title
human	WB,ChIP	Biol Direct; ELK4 induced upregulation of HOMER3 promotes the proliferation and metastasis in glioma via Wnt/β-catenin/EMT signaling pathway; Authors - Furong Wang
human	WB	Front Oncol; tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway; Authors - Weiguo Xu
mouse	WB	J Pers Med; Exosomes from Microvascular Endothelial Cells under Mechanical Unloading Inhibit Osteogenic Differentiation via miR-92b-3p/ELK4 Axis; Authors - Xiaoyan Zhang
mouse	WB	Brain Res Bull; ELK4 ameliorates cognitive impairment and neuroinflammation induced by obstructive sleep apnea; Authors - Haiming Yang

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