Anticorps Polyclonal de lapin anti-ELL

• Phare
• Validé par KD/KO

ELL Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 51044-1-AP

Synonymes

C19orf17, ELL, ELL1, MEN

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Galerie de données de validation

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  WB analysis of HeLa using 51044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 51044-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 51044-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 51044-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 51044-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51044-1-AP (ELL antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HL-60, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

51044-1-AP cible ELL dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELL Protéine recombinante Ag0617
• Nom complet: elongation factor RNA polymerase II
• Masse moléculaire calculée: 500 aa, 68 kDa
• Poids moléculaire observé: 68-80 kDa
• Numéro d’acquisition GenBank: BC049195
• Symbole du gène: ELL
• Identification du gène (NCBI): 8178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun; Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.; Authors - Chia-Yu Chung ; KD Validated
human	WB	Nat Commun; ELL targets c-Myc for proteasomal degradation and suppresses tumour growth.; Authors - Yu Chen
mouse	WB	Oncogene; The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.; Authors - Hao Wang
human	WB	Prostate; ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.; Authors - Liu Lingqi L ; KD Validated
human	WB	Mol Immunol; Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.; Authors - Lou-Ella M M Alexander ; KD Validated
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao