Anticorps Polyclonal de lapin anti-Elastin

Elastin Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 15257-1-AP

Synonymes

desmosine, elastin, ELN, SVAS, Tropoelastin, WBS, WS

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Galerie de données de validation

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  WB analysis using 15257-1-AP

  Human artery tissue were subjected to SDS PAGE followed by western blot with 15257-1-AP (Elastin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 15257-1-AP

  IP result of anti-Elastin (IP:15257-1-AP, 4ug; Detection:15257-1-AP 1:300) with human placenta tissue lysate 4000 ug.

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  IHC staining of human lung using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu artériel humain
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

15257-1-AP cible Elastin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Elastin Protéine recombinante Ag7179
• Nom complet: elastin
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC065566
• Symbole du gène: ELN
• Identification du gène (NCBI): 2006
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Elastic fibers are an abundant and integral part of many extracellular matrices, in which they provide the elastic properties to tissues such as arterial, lung, and skin. Elastic fibers are consisting of an elastin core surrounded by a mantle of fibrillin-rich microfibrils (PMID: 12082143). Elastin is an extremely durable, insoluble biopolymer formed through the lysine-mediated crosslinking of its soluble precursor tropoelastin, which is an approximately 60-70 kDa protein (PMID: 15837523). Deletions and mutations in the elastin gene (ELN) are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.

Publications

Species	Application	Title
human	WB	Am J Respir Cell Mol Biol; Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation.; Authors - Jing Ge
human	WB	Int J Mol Sci; αvβ3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis; Authors - Maia Yamila Kohon
		J Cell Physiol; Novel insight into the role of clusterin on intraocular pressure regulation by modifying actin polymerization and extracellular matrix remodeling in the trabecular meshwork.; Authors - Avinash Soundararajan
human	WB	Cells; Cathepsin K Regulates Intraocular Pressure by Modulating Extracellular Matrix Remodeling and Actin-Bundling in the Trabecular Meshwork Outflow Pathway.; Authors - Avinash Soundararajan
human	WB	Front Pharmacol; The Extracellular Matrix Enriched With Exosomes for the Treatment on Pulmonary Fibrosis in Mice; Authors - Yanzhen Yu
	WB	Am J Physiol Cell Physiol; miR-29c Induction Contributes to Down-Regulation of Vascular Extracellular Matrix Proteins by Glucocorticoids.; Authors - Tsai-Der Chuang

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