Anticorps Polyclonal de lapin anti-EMR1

EMR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 27044-1-AP

Synonymes

ADGRE1, Adhesion G protein-coupled receptor E1, EGF like module receptor 1, EMR1, EMR1 hormone receptor, F4/80, TM7LN3

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 27044-1-AP

  unboiled RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27044-1-AP (EMR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 27044-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27044-1-AP (EMR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 27044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27044-1-AP (EMR1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27044-1-AP (EMR1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27044-1-AP (EMR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27044-1-AP (EMR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27044-1-AP (EMR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse peritoneal macrophages using 27044-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed mouse peritoneal macrophages using EMR1 antibody (27044-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: unboiled RAW 264.7 cells, cellules HL-60
• Résultats positifs en IHC: tissu splénique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: macrophages péritonéaux de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 18 publications below
• IF: See 7 publications below

Informations sur le produit

27044-1-AP cible EMR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMR1 Protéine recombinante Ag25883
• Nom complet: egf-like module containing, mucin-like, hormone receptor-like 1
• Masse moléculaire calculée: 886 aa, 97 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC059395
• Symbole du gène: EMR1
• Identification du gène (NCBI): 2015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EMR1 (EGF-like module containing mucin-like hormone receptor 1), also known as Adhesion G protein-coupled receptor E1 (ADGRE1), is a surface receptor with seven transmembrane segments that belong to the EGF-7-transmembrane family of G protein-coupled receptors (PMID: 14647991, 7601460). EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans and may be used as a novel therapeutic target for eosinophilic disorders (PMID: 17823986, 24530099). F4/80, the murine homolog of EMR1, is a marker of murine macrophage. The apparent molecular weight of F4/80 is 160 kDa, which is larger than the calculated molecular weight due to post-translational modifications (PMID: 7308288; 8647179).

Publications

Species	Application	Title
human	IHC	Cell Res; A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling.; Authors - Rui-Hua Li
mouse	IHC	Acta Pharm Sin B; A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responses; Authors - Yuhan Li
human	IHC	Theranostics; SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.; Authors - Ling Lin
human	IF	Cancer Lett; MicroRNA-15b in extracellular vesicles from arsenite-treated macrophages promotes the progression of hepatocellular carcinomas by blocking the LATS1-mediated Hippo pathway.; Authors - Junjie Li
human	IHC	Front Immunol; PMID: 31249571; Authors - Le Liu
pig	IHC	Biomed Pharmacother; A systematic comparison of the effect of topically applied anthraquinone aglycones to relieve psoriasiform lesion: The evaluation of percutaneous absorption and anti-inflammatory potency.; Authors - Chwan-Fwu Lin