Anticorps Polyclonal de lapin anti-Endoglin/CD105

• Phare
• Validé par KD/KO

Endoglin/CD105 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(55)

N° de cat : 10862-1-AP

Synonymes

CD105, END, Endoglin, ENG, HHT1

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Galerie de données de validation

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  WB analysis of HeLa using 10862-1-AP

  WB result of Endoglin/CD105 antibody (10862-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Endoglin/CD105 transfected HeLa cells.

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  WB analysis using 10862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10862-1-AP

  human placenta tissue was subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10862-1-AP

  IP result of anti-Endoglin/CD105 (IP:10862-1-AP, 4ug; Detection:10862-1-AP 1:2000) with mouse lung tissue lysate 1760 ug.

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  IHC staining of human breast cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, tissu placentaire humain, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu placentaire humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 13 publications below
• IF: See 19 publications below
• IP: See 1 publications below

Informations sur le produit

10862-1-AP cible Endoglin/CD105 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Endoglin/CD105 Protéine recombinante Ag1287
• Nom complet: endoglin
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 75-90 kDa, 170-180 kDa
• Numéro d’acquisition GenBank: BC014271
• Symbole du gène: CD105
• Identification du gène (NCBI): 2022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publications

Species	Application	Title
rat	IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	Dev Cell; Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation; Authors - Bin Tan
rat		Stem Cell Res Ther; Controlled release of hydrogel-encapsulated mesenchymal stem cells-conditioned medium promotes functional liver regeneration after hepatectomy in metabolic dysfunction-associated steatotic liver disease; Authors - Naoya Kasahara
human	WB	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou
human	WB	Biomedicines; Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.; Authors - Mahendra Jadhao ; KD Validated

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