Anticorps Monoclonal anti-ENO1

• Phare
• Validé par KD/KO

ENO1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1D6E8

N° de cat : 67187-1-Ig

Synonymes

1D6E8, 2-phospho-D-glycerate hydro-lyase, Alpha-enolase, C-myc promoter-binding protein, EC:4.2.1.11

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Galerie de données de validation

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  WB analysis of HEK-293 using 67187-1-Ig

  WB result of ENO1 antibody (67187-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells.

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  WB analysis using 67187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67187-1-Ig (ENO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 67187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules PC-3, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67187-1-Ig cible ENO1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ENO1 Protéine recombinante Ag17518
• Nom complet: enolase 1, (alpha)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015641
• Symbole du gène: ENO1
• Identification du gène (NCBI): 2023
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)

Publications

Species	Application	Title
human	WB	Tissue Cell; Activation of mTOR/HK2 signaling mitigates effects of PYCR2 depletion in colorectal cells; Authors - Li Chen

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