Anticorps Polyclonal de lapin anti-NSE/ENO2-specific

• Phare
• Validé par KD/KO

NSE/ENO2-specific Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 55235-1-AP

Synonymes

Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE, NSE/ENO2-specific

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Galerie de données de validation

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  WB analysis of U-251 using 55235-1-AP

  WB result of NSE/ENO2-specific antibody (55235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE/ENO2-specific transfected U-251 cells.

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  WB analysis of SH-SY5Y using 55235-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55235-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55235-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55235-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HEK-293, cellules HepG2, cellules U-251, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

55235-1-AP cible NSE/ENO2-specific dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: enolase 2 (gamma, neuronal)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: NM_001975
• Symbole du gène: ENO2
• Identification du gène (NCBI): 2026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. This antibody is specific to ENO2 and has no cross reaction to ENO1 and ENO3.

Publications

Species	Application	Title
mouse	WB	Mol Med Rep; miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase.; Authors - Chunli Xu