Anticorps Polyclonal de lapin anti-ENO3-specific

• Phare
• Validé par KD/KO

ENO3-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 55234-1-AP

Synonymes

Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

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Galerie de données de validation

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  WB analysis using 55234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55234-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55234-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 55234-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55234-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55234-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55234-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

55234-1-AP cible ENO3-specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: enolase 3 (beta, muscle)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 42-47 kDa
• Numéro d’acquisition GenBank: NM_001976
• Symbole du gène: ENO3
• Identification du gène (NCBI): 2027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

Publications

Species	Application	Title
bovine	WB	J Proteomics; Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation.; Authors - Turner H Swartz
human	WB	Ann Transl Med; ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation.; Authors - Di Lu ; KD Validated
human	WB,IHC	Med Oncol; ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.; Authors - Jingyu Chen ; KD Validated
	WB	Reprod Domest Anim; Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.; Authors - M D Xi