Anticorps Polyclonal de lapin anti-CD39/ENTPD1

CD39/ENTPD1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14211-1-AP

Synonymes

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

---

Galerie de données de validation

• 

  WB analysis of mouse placenta using 14211-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (ENTPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14211-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14211-1-AP

  human placenta lysates were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14211-1-AP

  Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14211-1-AP

  Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 14211-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (14211-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: tissu placentaire de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14211-1-AP cible CD39/ENTPD1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD39/ENTPD1 Protéine recombinante Ag5429
• Nom complet: ectonucleoside triphosphate diphosphohydrolase 1
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 50 kDa，70-100 kDa
• Numéro d’acquisition GenBank: BC047664
• Symbole du gène: ENTPD1
• Identification du gène (NCBI): 953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB,IF	Int J Biol Sci; CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence; Authors - Zhenni Liu
human	IF	Genomics Proteomics Bioinformatics; Single-cell Immune Landscape of Human Recurrent Miscarriage.; Authors - Feiyang Wang
human	IHC	Oncotarget; Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.; Authors - Federica Costa
rat	IHC	Am J Physiol Gastrointest Liver Physiol; Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.; Authors - Moyuru Hayashi
human	WB	BMC Med Genomics; Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.; Authors - Xing-Cheng Zhao
rat	WB	J Gerontol A Biol Sci Med Sci; Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling; Authors - Lori A Birder