Anticorps Polyclonal de lapin anti-CD39/ENTPD1

CD39/ENTPD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 19229-1-AP

Synonymes

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

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Galerie de données de validation

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  WB analysis of Jurkat using 19229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 19229-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 19229-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19229-1-AP

  IP Result of anti-CD39 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsillitis using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsillitis using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human placenta using 19229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 19229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 19229-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD39 antibody (19229-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, tissu cérébral de souris, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu placentaire humain,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

19229-1-AP cible CD39/ENTPD1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD39/ENTPD1 Protéine recombinante Ag6165
• Nom complet: ectonucleoside triphosphate diphosphohydrolase 1
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 75-95 kDa
• Numéro d’acquisition GenBank: BC047664
• Symbole du gène: ENTPD1
• Identification du gène (NCBI): 953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478aa) molecule.

Publications

Species	Application	Title
mouse	WB	Bone Res; Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.; Authors - Wenjia Liu
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
human	FC	J Chem Inf Model; Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays.; Authors - Yunshuo Zhao
rat	WB	Biochem J; Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.; Authors - Liziane Raquel Beckenkamp
mouse	IHC	JCI Insight; Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.; Authors - Kan Zhang
mouse	WB,IHC	Cancer Immunol Immunother; STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma; Authors - Hao Cui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Isabelle (Verified Customer) (10-08-2020)

good antibody for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: human liver, hepG2 cell line, renal tubular HK2 cell line