Anticorps Polyclonal de lapin anti-ENTPD3

ENTPD3 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 13021-1-AP

Synonymes

Ecto-ATPase 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, EC:3.6.1.5, CD39L3

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Galerie de données de validation

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  WB analysis of human brain using 13021-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13021-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13021-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 13021-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ENTPD3 antibody (13021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules Jurkat, cellules Raji
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13021-1-AP cible ENTPD3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENTPD3 Protéine recombinante Ag4114
• Nom complet: ectonucleoside triphosphate diphosphohydrolase 3
• Masse moléculaire calculée: 529 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC029869
• Symbole du gène: ENTPD3
• Identification du gène (NCBI): 956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENTPD3, also known as NTPDase3 or CD39L3, belongs to the GDA1/CD39 NTPase family. ENTPD3 has a threefold preference for the hydrolysis of ATP over ADP. ENTPD3 is expressed in adult brain, pancreas, spleen and prostate, but not in liver and peripheral blood leukocytes. ENTPD3 has 2 isoforms with the molecular mass of 51 and 59 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice.; Authors - Qian Wang
mouse	WB	Reprod Sci; Expression and Hormonal Regulation of Entpd3 at Various Estrous Cycle Stages in the Mouse Uterus; Authors - Dan Mo

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