Anticorps Polyclonal de lapin anti-EphA4

• Phare
• Validé par KD/KO

EphA4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 21875-1-AP

Synonymes

EK8, EPH like kinase 8, EPH receptor A4, EPHA4, Ephrin type A receptor 4, HEK8, SEK, TYRO1, Tyrosine protein kinase TYRO1

---

Galerie de données de validation

• 

  WB analysis using 21875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21875-1-AP (EphA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21875-1-AP

  IP Result of anti-EphA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 21875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuron cells using 21875-1-AP

  IF result of EphA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou.

• 

  FC experiment of SH-SY5Y using 21875-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug EphA4 antibody (21875-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: Cellules neuronales
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 2 publications below

Informations sur le produit

21875-1-AP cible EphA4 dans les applications de WB, IP, IHC, IF, FC, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EphA4 Protéine recombinante Ag16233
• Nom complet: EPH receptor A4
• Masse moléculaire calculée: 986 aa, 110 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC026327
• Symbole du gène: EPHA4
• Identification du gène (NCBI): 2043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EphA4 is a member of the Eph receptor tyrosine kinase family and has important functions in the developing and adult nervous system (PMID: 14697664). The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). EphA4 is involved in commissure formation within the forebrain, axonal guidance in the corticospinal tract, regulation of the central pattern generator that provides normal locomotor function and axonal regeneration following spinal cord injury (PMID: 30061574). EphA4 has been implicated as a disease modifier of amyotrophic lateral sclerosis (ALS) (PMID: 22922411).

Publications

Species	Application	Title
mouse	WB	Nat Commun; EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment.; Authors - Shilpa Bhatia
mouse	IHC	Proc Natl Acad Sci U S A; Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity; Authors - Alison Cash
human	WB,IHC	Biochem Biophys Res Commun; Single-chain fragment antibody disrupting the EphA4 function as a therapeutic drug for gastric cancer; Authors - Chen-Wei Chiang
rat	WB	Brain Res Bull; Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats; Authors - Tao Tong
human	IF	bioRxiv; Identification of new ciliary signaling pathways in the brain and insights into neurological disorders; Authors - Abdelhalim Loukil
mouse	FC	JCI Insight; Monocyte pro-inflammatory phenotypic control by Ephrin type-A receptor 4 mediates neural tissue damage.; Authors - Elizabeth A Kowalski

Produits associés

ERK1/2 Polyclonal antibody

11257-1-AP

Ephrin A3 Polyclonal antibody

12480-1-AP

CDK5 Polyclonal antibody

10430-1-AP

NGEF Polyclonal antibody

13271-1-AP

RAC1 Monoclonal antibody

66122-1-Ig