Anticorps Polyclonal de lapin anti-EPSTI1

• Phare
• Validé par KD/KO

EPSTI1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 11627-1-AP

Synonymes

Epithelial-stromal interaction protein 1

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Galerie de données de validation

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  WB analysis using 11627-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 11627-1-AP (EPSTI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11627-1-AP

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 11627-1-AP (EPSTI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

11627-1-AP cible EPSTI1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPSTI1 Protéine recombinante Ag2157
• Nom complet: epithelial stromal interaction 1 (breast)
• Masse moléculaire calculée: 410 aa, 47 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC023660
• Symbole du gène: EPSTI1
• Identification du gène (NCBI): 94240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Real-time PCR revealed the highest expression in the placenta; strong expression in the small intestine, spleen, salivary gland, and testes; and low expression levels in normal breast and several other tissues. The expression of EPSTI1 was upregulated (range 5.6-72.1) in all 14 breast carcinomas tested. Microdissection revealed that both tumor and stromal tissue overexpressed EPSTI1. (PubMed: 11991720)

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Acta Pharmacol Sin; EPSTI1 promotes monocyte adhesion to endothelial cells in vitro via upregulating VCAM-1 and ICAM-1 expression.; Authors - Yan-Rou Bei ; KD Validated
human	WB	Elife; KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition.; Authors - Chaitra Rao
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB	Mol Cancer; Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.; Authors - Erfei Chen
human	IHC	Oxid Med Cell Longev; Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer; Authors - Mujing Ke

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