Anticorps Polyclonal de lapin anti-HER2/ErbB2

• Phare
• Validé par KD/KO

HER2/ErbB2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

56

Avis (1)

N° de cat : 18299-1-AP

Synonymes

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

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Galerie de données de validation

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  WB analysis using 18299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18299-1-AP

  WB result of HER2/ErbB2 antibody (18299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HER2/ErbB2 transfected MCF-7 cells.

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  WB analysis of SGC-7901 using 18299-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SGC-7901 using 18299-1-AP

  IP Result of anti-HER2/ErbB2 (IP:18299-1-AP, 5ug; Detection:18299-1-AP 1:500) with SGC-7901 cells lysate 2400ug.

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  IHC staining of human breast cancer using 18299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 18299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 18299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 18299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of human breast cancer using 18299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 18299-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug HER2/ErbB2 antibody (18299-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules 4T1, cellules HeLa, cellules MCF-7, cellules SGC-7901
• Résultats positifs en IP: cellules SGC-7901
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 43 publications below
• IHC: See 12 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

18299-1-AP cible HER2/ErbB2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 138 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC167147
• Symbole du gène: ERBB2
• Identification du gène (NCBI): 2064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.

Publications

Species	Application	Title
human	IHC	Cell Res; Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers.; Authors - Daisong Wang
mouse	WB	Small; Efficient Delivery of GSDMD-N mRNA by Engineered Extracellular Vesicles Induces Pyroptosis for Enhanced Immunotherapy; Authors - Yuqi Xing
human	IHC	Nat Commun; Targeting 17q23 amplicon to overcome the resistance to anti-HER2 therapy in HER2+ breast cancer.; Authors - Yunhua Liu
human	WB	J Immunother Cancer; Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells.; Authors - Shasha Zhao
human	WB	Cancer Res; Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer.; Authors - Jiao Wang
human	WB,FC	ACS Sens; Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.; Authors - Xiaoyi Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vanina (Verified Customer) (07-28-2021)

MDA-MB-453 cell line was transfected with different concentrations of HER2/Erbb2 siRNA for 48h. Western blot using HER2/Erbb2 rabbit Proteintech antibody (18299-1-AP) at 1:2,000 dillution confirms the HER2/Erbb2 knockdown.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: MDA-MB-453 breast cancer cell line

HER2/ErbB2 Antibody Western Blot validation (1:2,000 dilution) in MDA-MB-453 breast cancer cell line (Cat no:18299-1-AP)

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