Anticorps Monoclonal anti-HER2/ErbB2

HER2/ErbB2 Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1B12A7

Avis (1)

N° de cat : 60311-1-Ig

Synonymes

ERBB2, HER2, HER 2/neu, HER 2, CD340

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Galerie de données de validation

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  IHC staining of human breast cancer using 60311-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60311-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60311-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 60311-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 60311-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of SK-BR-3 using 60311-1-Ig

  1x10^6 SK-BR-3 cells were surface stained with 0.2 μg HER2/ErbB2 Monoclonal antibody (60311-1-Ig, Clone: 1B12A7) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4) was parallel stained as control. Cells were not fixed.

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,
• Résultats positifs en cytométrie: SK-BR-3 cells,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

60311-1-Ig cible HER2/ErbB2 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HER2/ErbB2 Protéine recombinante Ag16463
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 1255 aa, 138 kDa
• Numéro d’acquisition GenBank: BC156755
• Symbole du gène: HER2/ErbB2
• Identification du gène (NCBI): 2064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HER2, ErbB2, and Neu is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

Publications

Species	Application	Title
human	IHC	J Cancer; InvivoPen: A novel plasma source for in vivo cancer treatment.; Authors - Xin Zhou
human	IHC	Mol Med Rep; HPK1 positive expression associated with longer overall survival in patients with estrogen receptor-positive invasive ductal carcinoma‑not otherwise specified.; Authors - Jiaojiao Wang
human	IHC	Oncol Lett; Circulating HER-2 mRNA in the peripheral blood as a potential diagnostic and prognostic biomarker in females with breast cancer.; Authors - Yanlin Wu
human	IF	Mol Cancer; CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer; Authors - Fei Xing
human	IHC	Int J Cancer; Establishment of patient-derived organoids for guiding personalized therapies in breast cancer patients; Authors - Huizi Wu
human	IF	Adv Mater; Targeted SERS Imaging and Intraoperative Real-Time Elimination of Microscopic Tumors for Improved Breast-Conserving Surgery; Authors - Yu Wen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Felletár (Verified Customer) (06-25-2025)

Immunostaining was performed using an automated staining, covering, and scanning system. FFPE slides were incubated for 30 minutes. HRP conjugated one step development system, scanning under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:5000 ( 30min )
• Cell Tissue Type: Human breast carces tissue

HER2/ErbB2 Antibody Immunohistochemistry validation (1:5000 ( 30min ) dilution) in Human breast carces tissue (Cat no:60311-1-Ig)

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