Anticorps Polyclonal de lapin anti-ERBB3

ERBB3 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 10369-1-AP

Synonymes

c erbB 3, c erbB3, ErbB 3, ERBB3, erbB3 S, HER3, LCCS2, MDA BF 1, p180 ErbB3, p45 sErbB3, p85 sErbB3

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Galerie de données de validation

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  IHC staining of human breast cancer using 10369-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10369-1-AP (ERBB3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10369-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10369-1-AP (ERBB3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 10369-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10369-1-AP (ERBB3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 10369-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10369-1-AP (ERBB3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below

Informations sur le produit

10369-1-AP cible ERBB3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERBB3 Protéine recombinante Ag0215
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• Masse moléculaire calculée: 148 kDa
• Numéro d’acquisition GenBank: BC002706
• Symbole du gène: ERBB3
• Identification du gène (NCBI): 2065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. These family members form either homodimers or heterodimers upon ligand binding to mediate cell growth. ErbB-3 binds and is activated by neuregulins and NTAK. It forms a heterodimer with each of the other ErbB receptors. ErbB-3 is predominantly expressed in epithelial tissues and brain, and is overexpressed in a subset of human mammary tumors.

Publications

Species	Application	Title
human	WB	Acta Pharmacol Sin; Quantitative systems pharmacology modeling of HER2-positive metastatic breast cancer for translational efficacy evaluation and combination assessment across therapeutic modalities; Authors - Ya-Ting Zhou
human	WB	Oncotarget; Tumor suppressor role of miR-3622b-5p in ERBB2-positive cancer.; Authors - Mingjie Lu
human	WB	Cells; NOX4 Signaling Mediates Cancer Development and Therapeutic Resistance through HER3 in Ovarian Cancer Cells.; Authors - Wen-Jing Liu
human	WB	J Oncol; CircEPSTI1 Promotes the Proliferation of HER2-Positive Breast Cancer Cells via circEPSTI1/miR-145/ERBB3 Axis; Authors - Yue Zhang
human	IHC	Acta Otolaryngol; ErbB1 and ErbB2 overexpression in patients with sinonasal inverted papilloma and inverted papilloma with squamous cell carcinoma in China.; Authors - Hongbing Li
human,mouse	WB	Front Oncol; NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes; Authors - Ruobing Leng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgan (Verified Customer) (10-26-2021)

I could not get any detection for this antibody at all. It strangely appeared on the ladder and I did not know how to troubleshoot. It occurred in both western blot attempts that I tried (Please see images)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: OV90 Ovarian Cancer Cells

ERBB3 Antibody Western Blot validation (1:500 dilution) in OV90 Ovarian Cancer Cells (Cat no:10369-1-AP)

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