Anticorps Polyclonal de lapin anti-ERC1

• Phare
• Validé par KD/KO

ERC1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 22211-1-AP

Synonymes

Cast2, ELKS, ERC 1, ERC1, KIAA1081, Rab6 interacting protein 2, RAB6IP2

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Galerie de données de validation

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  WB analysis of HeLa using 22211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22211-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 22211-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 22211-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ERC1 antibody 22211-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules Jurkat, tissu testiculaire humain
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22211-1-AP cible ERC1 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERC1 Protéine recombinante Ag17536
• Nom complet: ELKS/RAB6-interacting/CAST family member 1
• Masse moléculaire calculée: 1116 aa, 128 kDa
• Poids moléculaire observé: 124-135 kDa
• Numéro d’acquisition GenBank: BC132782
• Symbole du gène: ERC1
• Identification du gène (NCBI): 23085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).

Publications

Species	Application	Title
	WB	Sci Rep; Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry.; Authors - Qingyang Liu
rat	WB,CoIP	J Cell Sci; Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells.; Authors - Ivar Noordstra
human	WB	J Biol Chem; MICAL3 flavoprotein monooxygenase forms a complex with centralspindlin and regulates cytokinesis.; Authors - Qingyang Liu ; KD Validated
human	IF	J Cell Biol; RAB6 and microtubules restrict protein secretion to focal adhesions.; Authors - Lou Fourriere

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Ab performs well by Immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 200 IF
• Cell Tissue Type: HUVEC

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