Anticorps Monoclonal anti-ERK1/2

ERK1/2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

26

CloneNo.

4G8A11

N° de cat : 66192-1-Ig

Synonymes

ERK, ERK 1, ERK 2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Galerie de données de validation

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  WB analysis using 66192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66192-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66192-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66192-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human cervical cancer using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IF Staining of HepG2 using 66192-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2000-1:6000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• CoIP: See 1 publications below

Informations sur le produit

66192-1-Ig cible ERK1/2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERK1/2 Protéine recombinante Ag20350
• Nom complet: mitogen-activated protein kinase 3
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC013992
• Symbole du gène: MAPK3
• Identification du gène (NCBI): 5595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
mouse	CoIP	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
human	WB	Oncogene; ALK fusion promotes metabolic reprogramming of cancer cells by transcriptionally upregulating PFKFB3; Authors - Mengnan Hu
human	WB	Stem Cells; miR-223 regulates adipogenic and osteogenic differentiation of mesenchymal stem cells through a C/EBPs/miR-223/FGFR2 regulatory feedback loop.; Authors - Xiaohui Guan
mouse	WB	Diabetes; Pygo2 Regulates Adiposity and Glucose Homeostasis via β-Catenin-Axin2-GSK3β Signaling Pathway.; Authors - Yuan-Yuan Xie
human	WB	Biomater Sci; mTORC1-dependent TFEB nucleus translocation and pro-survival autophagy induced by zeolitic imidazolate framework-8.; Authors - He Ding
human	WB	Pharmacol Res; Anti-thrombotic effects mediated by dihydromyricetin involve both platelet inhibition and endothelial protection.; Authors - Shuai Chen

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