Anticorps Polyclonal de lapin anti-ERK1/2

• Phare
• Validé par KD/KO

ERK1/2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

231

Avis (1)

N° de cat : 11257-1-AP

Synonymes

ERK, ERK 1, ERK 1/2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Galerie de données de validation

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  WB analysis using 11257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.

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  WB analysis of HeLa using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.

• 

  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 11257-1-AP

  Immunofluorescent analysis of A375 cells using 11257-1-AP (ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 11257-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11257-1-AP (ERK1/2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11257-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C6, cellules HEK-293, cellules HeLa, cellules PC-12, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A375, cellules MCF-7
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 227 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Informations sur le produit

11257-1-AP cible ERK1/2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERK1/2 Protéine recombinante Ag1759
• Nom complet: mitogen-activated protein kinase 3
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC013992
• Symbole du gène: MAPK3
• Identification du gène (NCBI): 5595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
	WB	Cancer Res; Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells; Authors - Li Xia
mouse	WB	ACS Appl Mater Interfaces; Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.; Authors - Chaoqun Li
mouse	WB	J Nanobiotechnology; Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis; Authors - Ni Yang
human	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
human	WB	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu
	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

XB (Verified Customer) (10-29-2020)

A reliable ab to detect total ERK1/2 by Western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain

ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)