Anticorps Polyclonal de lapin anti-ERLIN1

ERLIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17311-1-AP

Synonymes

C10orf69, ER lipid raft associated 1, Erlin 1, ERLIN1, KE04, KEO4, Protein KE04, SPFH1

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Galerie de données de validation

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  WB analysis using 17311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human ovary cancer using 17311-1-AP

  human ovary cancer were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 17311-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17311-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17311-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17311-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17311-1-AP

  IP Result of anti-ERLIN1 (IP:17311-1-AP, 3ug; Detection:17311-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse liver using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17311-1-AP

  Immunofluorescent analysis of HeLa cells using 17311-1-AP (ERLIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules COLO 320, cellules U-251, tissu cérébral humain, tissu de cancer de l'ovaire humain, tissu hépatique de souris, tissu pancréatique de rat, tissu rénal humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

17311-1-AP cible ERLIN1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERLIN1 Protéine recombinante Ag11279
• Nom complet: ER lipid raft associated 1
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC031791
• Symbole du gène: ERLIN1
• Identification du gène (NCBI): 10613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell; BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.; Authors - Fabien Llambi
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
human	WB	Elife; RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells.; Authors - Kirsteen M Tullett
human	WB	PLoS Genet; Disruption of the ERLIN-TM6SF2-APOB complex destabilizes APOB and contributes to non-alcoholic fatty liver disease.; Authors - Bo-Tao Li