Anticorps Polyclonal de lapin anti-TXNDC4

• Phare
• Validé par KD/KO

TXNDC4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP

Conjugaison

Non conjugué

Publications

5

N° de cat : 16016-1-AP

Synonymes

ER protein 44, ERP44, KIAA0573, PDIA10, TXNDC4

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Galerie de données de validation

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  WB analysis using 16016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16016-1-AP

  WB result of TXNDC4 antibody (16016-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC4 transfected HepG2 cells.

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  WB analysis of K-562 using 16016-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16016-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16016-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16016-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16016-1-AP

  IP Result of anti-TXNDC4 (IP:16016-1-AP, 4ug; Detection:16016-1-AP 1:500) with K-562 cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16016-1-AP

  Immunofluorescent analysis of Hela cells, using ERP44 antibody 16016-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

16016-1-AP cible TXNDC4 dans les applications de WB, IP, IHC, IF, CoIP et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TXNDC4 Protéine recombinante Ag8818
• Nom complet: endoplasmic reticulum protein 44
• Masse moléculaire calculée: 406 aa, 47 kDa
• Poids moléculaire observé: 44-47 kDa
• Numéro d’acquisition GenBank: BC005374
• Symbole du gène: ERP44
• Identification du gène (NCBI): 23071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.

Publications

Species	Application	Title
human	IF	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
human	WB,IF	Antioxid Redox Signal; Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM).; Authors - Anelli Tiziana T
human	WB,IHC,CoIP	J Transl Med; ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.; Authors - Hui Tian ; KD Validated
human	WB	Traffic; Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells.; Authors - Tiziana Anelli
human,mouse	WB,IHC	BMC Cancer; Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma; Authors - Tian Xia