Anticorps Polyclonal de lapin anti-ESD

• Phare
• Validé par KD/KO

ESD Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 15244-1-AP

Synonymes

S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12

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Galerie de données de validation

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  WB analysis of HAP1 using 15244-1-AP

  WB result of ESD antibody (15244-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and ESD knockout HAP1 cells.

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  WB analysis of K-562 using 15244-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15244-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, HAP1 cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15244-1-AP cible ESD dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESD Protéine recombinante Ag7457
• Nom complet: esterase D/formylglutathione hydrolase
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC001169
• Symbole du gène: ESD
• Identification du gène (NCBI): 2098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

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