Anticorps Polyclonal de lapin anti-ESRP1

• Phare
• Validé par KD/KO

ESRP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 21045-1-AP

Synonymes

ESRP1, RBM35A, RNA binding motif protein 35A, RNA binding protein 35A

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Galerie de données de validation

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  WB analysis of MCF-7 using 21045-1-AP

  WB result of ESRP1 antibody (21045-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ESRP1 transfected MCF-7 cells.

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  WB analysis of COLO 320 using 21045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21045-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 21045-1-AP

  IP Result of anti-ESRP1 (IP:21045-1-AP, 4ug; Detection:21045-1-AP 1:300) with COLO 320 cells lysate 4000ug.

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  IHC staining of mouse colon using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules MCF-7, tissu pulmonaire de souris
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

21045-1-AP cible ESRP1 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESRP1 Protéine recombinante Ag15386
• Nom complet: epithelial splicing regulatory protein 1
• Masse moléculaire calculée: 681 aa, 76 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC099916
• Symbole du gène: ESRP1
• Identification du gène (NCBI): 54845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ESRP1, also named as Epithelial splicing regulatory protein 1, is a 681 amino acid protein, which belongs to the ESRP family. ESRP1 as a mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. ESRP1 specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). ESRP1 exists various isoforms and molecular weight of each isoform is 76 kDa, 67 kDa, and 73 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; ESRP1 controls biogenesis and function of a large abundant multiexon circRNA; Authors - Dawei Liu ; KD Validated
	RIP	Mol Ther; circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis.; Authors - Chi Zhou ; KD Validated
human	WB	Cell Death Differ; Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop.; Authors - Wei Zhao
human		Oncogene; Androgen receptor signaling regulates the transcriptome of prostate cancer cells by modulating global alternative splicing.; Authors - Kalpit Shah
human	WB	Elife; Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.; Authors - Christina R Adams
human	WB,IF	Cell Signal; Silencing ESRP1 expression promotes caspase-independent cell death via nuclear translocation of AIF in colon cancer cells.; Authors - Yellamandayya Vadlamudi ; KD Validated