Anticorps Polyclonal de lapin anti-ESRRB

• Phare
• Validé par KD/KO

ESRRB Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 22644-1-AP

Synonymes

DFNB35, ERR2, ERRb, ERRbeta, ERRbeta 2, ESRL2, ESRRB, ESRRB protein, estrogen related receptor beta, NR3B2

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Galerie de données de validation

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  WB analysis of PC-13 using 22644-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22644-1-AP (ESRRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22644-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22644-1-AP (ESRRB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 22644-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22644-1-AP (ESRRB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22644-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22644-1-AP (ESRRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 22644-1-AP

  IP Result of anti-ESRRB (IP:22644-1-AP, 4ug; Detection:22644-1-AP 1:300) with A549 cells lysate 1040ug.

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Applications testées

• Résultats positifs en WB: cellules PC-13, cellules A549
• Résultats positifs en IP: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

22644-1-AP cible ESRRB dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESRRB Protéine recombinante Ag18523
• Nom complet: estrogen-related receptor beta
• Masse moléculaire calculée: 508 aa, 56 kDa
• Poids moléculaire observé: 48-56 kDa
• Numéro d’acquisition GenBank: BC131517
• Symbole du gène: ESRRB
• Identification du gène (NCBI): 2103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	IF	Nucleic Acids Res; The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity.; Authors - Stephanie Chrysanthou
human	WB,IF	Genes Dev; Role of the small subunit processome in the maintenance of pluripotent stem cells.; Authors - Kwon Tae You
mouse	WB,IF	Front Cell Dev Biol; ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.; Authors - Shuai Yu ; KD Validated
mouse	WB	ACS Nano; Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.; Authors - Jie Zhang
human	WB	Nat Commun; Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.; Authors - J Mathieu ; KO Validated

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