Anticorps Polyclonal de lapin anti-ESRRG

• Phare
• Validé par KD/KO

ESRRG Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 14017-1-AP

Synonymes

Estrogen-related receptor gamma, Estrogen receptor-related protein 3, ERRG2, ERR3, ERR gamma-2

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Galerie de données de validation

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  WB analysis of human spleen using 14017-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 14017-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14017-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique humain, tissu rénal humain
• Résultats positifs en IHC: tissu d'estomac de rat, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14017-1-AP cible ESRRG dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESRRG Protéine recombinante Ag5127
• Nom complet: estrogen-related receptor gamma
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC064700
• Symbole du gène: ESRRG
• Identification du gène (NCBI): 2104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen-related receptor gamma (ERR-gamma), also known as NR3B3 (nuclear receptor subfamily 3, group B, member 3), is a member of nuclear hormone receptor family of steroid hormone receptors. As an orphan nuclear receptor, it binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. It behaves as a constitutive activator of transcription. 4-hydroxytamoxifen and diethylstilbestrol may act as inverse agonists and deactivate ESRRG. It also seems to be the target of bisphenol A. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ESRRG.

Publications

Species	Application	Title
human	IHC,IF	Acta Neuropathol Commun; Nucleolar stress in C9orf72 and sporadic ALS spinal motor neurons precedes TDP-43 mislocalization.; Authors - Olubankole Aladesuyi Arogundade
human	WB	J Transl Med; ESRRG-PKM2 axis reprograms metabolism to suppress esophageal squamous carcinoma progression and enhance anti-PD-1 therapy efficacy; Authors - Tianxiao Wang
rat	WB	J Ethnopharmacol; Atractylodes macrocephala Rhizoma alleviates blood hyperviscosity induced by high-fat, high-sugar, and high-salt diet via inhibition of gut-liver inflammatory activation and fibrinogen synthesis; Authors - Qiwei Lin
human	WB	Mol Cancer Res; Integrative Epigenetic and Gene Expression Analysis of Renal Tumor Progression to Metastasis.; Authors - Hye-Young Nam
mouse	WB	Int J Biochem Cell Biol; Estrogen-related receptor (ERR) γ protects against puromycin aminonucleoside-induced podocyte apoptosis by targeting PI3K/Akt signaling.; Authors - Wei Gong ; KD Validated
mouse	WB	Front Pharmacol; Tanshinone IIA delays liver aging by modulating oxidative stress; Authors - Qi Liu

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