Anticorps Polyclonal de lapin anti-ETFA

ETFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12262-1-AP

Synonymes

Alpha ETF, Alpha-ETF, EMA, GA2, MADD

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Galerie de données de validation

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  WB analysis using 12262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 12262-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12262-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human ileum using 12262-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12262-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12262-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12262-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFA antibody (12262-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

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  FC experiment of MCF-7 using 12262-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug ETFA Polyclonal antibody (12262-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu d'iléon humain, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

12262-1-AP cible ETFA dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETFA Protéine recombinante Ag2931
• Nom complet: electron-transfer-flavoprotein, alpha polypeptide
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC015526
• Symbole du gène: ETFA
• Identification du gène (NCBI): 2108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.

Publications

Species	Application	Title
human	IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
human	WB	Bioorg Chem; Pomalidomide sensitizes lung cancer cells to TRAIL/CDDP-induced apoptosis via directly targeting electron transfer flavoprotein alpha subunit; Authors - Liangping Li
human	WB	J Proteome Res; Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor.; Authors - Han Bingnan B
human	WB	J Proteomics; Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis.; Authors - Muto Taika T

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