Anticorps Polyclonal de lapin anti-ETFB

ETFB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 17925-1-AP

Synonymes

Beta-ETF, FP585, MADD

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Galerie de données de validation

• 

  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17925-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17925-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17925-1-AP

  IP result of anti-ETFB (IP:17925-1-AP, 3ug; Detection:17925-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human stomach cancer using 17925-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17925-1-AP (ETFB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFB antibody (17925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

17925-1-AP cible ETFB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETFB Protéine recombinante Ag12344
• Nom complet: electron-transfer-flavoprotein, beta polypeptide
• Masse moléculaire calculée: 255 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC093961
• Symbole du gène: ETFB
• Identification du gène (NCBI): 2109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kDa alpha subunits (ETFA)and 28 kDa beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.It serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.ETFB has 2 isoforms produced by alternative splicing. Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B).

Publications

Species	Application	Title
human	IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Mol Cell; PrimPol, an archaic primase/polymerase operating in human cells.; Authors - Sara García-Gómez
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	Mol Cell Biol; A mutation in the FAD-dependent oxidoreductase FOXRED1 results in cell-type specific assembly defects in oxidative phosphorylation complexes I and II.; Authors - Olga Zurita Rendón
human	WB	J Biol Chem; Human METTL20 Methylates Lysine Residues Adjacent to the Recognition Loop of the Electron Transfer Flavoprotein in Mitochondria.; Authors - Virginie F Rhein
human	WB	J Cell Sci; Mitochondrial maintenance under oxidative stress depends on mitochondrial but not nuclear α isoform of OGG1.; Authors - Debora Lia

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