Anticorps Polyclonal de lapin anti-ETV4

• Phare
• Validé par KD/KO

ETV4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 10684-1-AP

Synonymes

E1A F, E1AF, ETS translocation variant 4, ets variant 4, ETV4, PEA3, PEAS3, Protein PEA3

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Galerie de données de validation

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  WB analysis of mouse heart using 10684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10684-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 10684-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10684-1-AP (ETV4 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

10684-1-AP cible ETV4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETV4 Protéine recombinante Ag0984
• Nom complet: ets variant 4
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC007242
• Symbole du gène: ETV4
• Identification du gène (NCBI): 2118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

Publications

Species	Application	Title
human	WB	Cell; Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.; Authors - Jaehong Suh
mouse	WB	Dev Cell; ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization.; Authors - Lee Yoontae Y ; KD Validated
mouse	WB	Nat Commun; Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.; Authors - Sungjun Park
human	WB	Cancer Res; Targeting pan-ETS factors inhibits melanoma progression.; Authors - Lee Huang
human,mouse	WB	Proc Natl Acad Sci U S A; Erk1/2 inactivation promotes a rapid redistribution of COP1 and degradation of COP1 substrates.; Authors - Weiming Ouyang
human	WB	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated