Anticorps Polyclonal de lapin anti-ETV5

• Phare
• Validé par KD/KO

ETV5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(29)

N° de cat : 13011-1-AP

Synonymes

Ets-related protein ERM, ETS translocation variant 5, Ets related protein ERM, ERM

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Galerie de données de validation

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  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 13011-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7, cellules PC-3, cellules Raji
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 12 publications below
• CoIP: See 1 publications below
• ChIP: See 5 publications below

Informations sur le produit

13011-1-AP cible ETV5 dans les applications de WB, IHC, IF/ICC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETV5 Protéine recombinante Ag3708
• Nom complet: ets variant 5
• Masse moléculaire calculée: 510 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007333
• Symbole du gène: ETV5
• Identification du gène (NCBI): 2119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

Publications

Species	Application	Title
human	WB	Cell; Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.; Authors - Jaehong Suh
human	ChIP	Nat Genet; The dynamic, combinatorial cis-regulatory lexicon of epidermal differentiation.; Authors - Daniel S Kim
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
mouse	IF	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
mouse	IF	Nat Commun; A critical role for NF2 and the Hippo pathway in branching morphogenesis.; Authors - Antoine Reginensi
mouse	IF, ChIP	Proc Natl Acad Sci U S A; Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.; Authors - Zhen Zhang

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