Anticorps Monoclonal anti-ETV5

ETV5 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, chIP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2A4A4

N° de cat : 66657-1-Ig

Synonymes

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5

---

Galerie de données de validation

• 

  WB analysis using 66657-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66657-1-Ig (ETV5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules T-47D, cellules DU 145, cellules HeLa, cellules LNCaP, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• ChIP: See 4 publications below

Informations sur le produit

66657-1-Ig cible ETV5 dans les applications de WB, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ETV5 Protéine recombinante Ag3708
• Nom complet: ets variant 5
• Masse moléculaire calculée: 510 aa, 58 kDa
• Poids moléculaire observé: 58-60 kDa
• Numéro d’acquisition GenBank: BC007333
• Symbole du gène: ETV5
• Identification du gène (NCBI): 2119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

Publications

Species	Application	Title
mouse	chIP	Elife; Etv transcription factors functionally diverge from their upstream FGF signaling in lens development.; Authors - Ankur Garg
mouse	chIP	PLoS Genet; FGF-induced Pea3 transcription factors program the genetic landscape for cell fate determination.; Authors - Ankur Garg
human	WB,ChIP	Cardiovasc Toxicol; ETV5-Mediated Transcriptional Repression of DDIT4 Blocks Macrophage Pro-Inflammatory Activation in Diabetic Atherosclerosis; Authors - Lili Shi
human	ChIP	J Ovarian Res; ANGPTL4 functions as an oncogene through regulation of the ETV5/CDH5/AKT/MMP9 axis to promote angiogenesis in ovarian cancer; Authors - Yinping Liu

{{ptg:RelatedPrimaryAntibodies}}