Anticorps Polyclonal de lapin anti-EXO1

• Phare
• Validé par KD/KO

EXO1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16253-1-AP

Synonymes

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI

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Galerie de données de validation

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  WB analysis using 16253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16253-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 16253-1-AP

  IP Result of anti-EXO1 (IP:16253-1-AP, 4ug; Detection:16253-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules Raji
• Résultats positifs en IP: tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below

Informations sur le produit

16253-1-AP cible EXO1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXO1 Protéine recombinante Ag9253
• Nom complet: exonuclease 1
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 115-120 kDa
• Numéro d’acquisition GenBank: BC007491
• Symbole du gène: EXO1
• Identification du gène (NCBI): 9156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.; Authors - Margaret Tammaro
human	WB	Nucleic Acids Res; PCNA promotes processive DNA end resection by Exo1.; Authors - Chen Xiaoqing X ; KD Validated
human	WB	Elife; Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations.; Authors - Birong Shen ; KD Validated
human	WB	PLoS Genet; EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.; Authors - Yuehan Wu
rat	WB	J Cardiovasc Transl Res; Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.; Authors - Xinliang Zou
human	WB	Front Genet; Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.; Authors - Christopher B Nelson ; KD Validated