Anticorps Polyclonal de lapin anti-SEC6

• Phare
• Validé par KD/KO

SEC6 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14703-1-AP

Synonymes

EXOC3, exocyst complex component 3, Exocyst complex component Sec6, SEC6, SEC6L1, Sec6p

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Galerie de données de validation

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  WB analysis of human brain using 14703-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14703-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14703-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14703-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14703-1-AP

  Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules COLO 320, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

14703-1-AP cible SEC6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC6 Protéine recombinante Ag6383
• Nom complet: exocyst complex component 3
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC064569
• Symbole du gène: EXOC3
• Identification du gène (NCBI): 11336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

Publications

Species	Application	Title
human	WB	Cell Microbiol; Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells.; Authors - Hiroki Takeuchi ; KD Validated
human	WB	Cell Microbiol; Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.; Authors - Takeuchi Hiroki H ; KD Validated
rat	WB	Dev Biol; Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition.; Authors - Youssef G G