Anticorps Polyclonal de lapin anti-EXOSC1

EXOSC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (2)

N° de cat : 12585-1-AP

Synonymes

CGI 108, CSL4, Csl4p, EXOSC1, exosome component 1, hCsl4p, p13, SKI4, Ski4p

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Galerie de données de validation

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  WB analysis of SGC-7901 using 12585-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 12585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 12585-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12585-1-AP

  IP result of anti-EXOSC1 (IP:12585-1-AP, 4ug; Detection:12585-1-AP 1:600) with HeLa cells lysate 3000 ug.

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  IHC staining of human lymphoma using 12585-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12585-1-AP (EXOSC1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901, cellules HeLa, cellules Jurkat, tissu d'estomac humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12585-1-AP cible EXOSC1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, levure
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC1 Protéine recombinante Ag3286
• Nom complet: exosome component 1
• Masse moléculaire calculée: 195 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC022067
• Symbole du gène: EXOSC1
• Identification du gène (NCBI): 51013
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Publications

Species	Application	Title
yeast	WB	Am J Med Genet A; Pontocerebellar hypoplasia associated with p.Arg183Trp homozygous variant in EXOSC1 gene: A case report; Authors - Nadirah S Damseh
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

EXOSC1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:12585-1-AP)

FH

Andrea (Verified Customer) (01-11-2023)

Great antibody! Were incubated overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:12585-1-AP)