Anticorps Polyclonal de lapin anti-EXOSC10

• Phare
• Validé par KD/KO

EXOSC10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 16731-1-AP

Synonymes

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

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Galerie de données de validation

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  WB analysis using 16731-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16731-1-AP

  WB result of EXOSC10 antibody (16731-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

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  WB analysis of mouse spleen using 16731-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16731-1-AP

  IP Result of anti-EXOSC10 (IP:16731-1-AP, 4ug; Detection:16731-1-AP 1:700) with HeLa cells lysate 1200ug.

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  IHC staining of human stomach cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16731-1-AP

  Immunofluorescent analysis of HeLa cells using 16731-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 16731-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 16731-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

16731-1-AP cible EXOSC10 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC10 Protéine recombinante Ag10189
• Nom complet: exosome component 10
• Masse moléculaire calculée: 885 aa, 101 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC073788
• Symbole du gène: EXOSC10
• Identification du gène (NCBI): 5394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Publications

Species	Application	Title
human	WB,IHC	PeerJ; EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification; Authors - Zhi-Yong Meng ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Antibody recognise Exosc10 band and some other non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:16731-1-AP)