Anticorps Polyclonal de lapin anti-EZH1

• Phare
• Validé par KD/KO

EZH1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 20852-1-AP

Synonymes

Enhancer of zeste homolog 1, ENX 2, EZH1, KIAA0388

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 20852-1-AP

  WB result of EZH1 antibody (20852-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH1 transfected RAW 264.7 cells.

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  WB analysis of HL-60 using 20852-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20852-1-AP (EZH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20852-1-AP

  HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 20852-1-AP (EZH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HEK-293T, cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

20852-1-AP cible EZH1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EZH1 Protéine recombinante Ag14732
• Nom complet: enhancer of zeste homolog 1 (Drosophila)
• Masse moléculaire calculée: 747 aa, 85 kDa
• Poids moléculaire observé: 80-95 kDa
• Numéro d’acquisition GenBank: BC015882
• Symbole du gène: EZH1
• Identification du gène (NCBI): 2145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human,chicken	WB,IF	Nat Commun; Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disorders; Authors - Carolina Gracia-Diaz
mouse	WB	Oxid Med Cell Longev; The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.; Authors - Yichao Du
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
mouse	WB	Drug Discov Ther; Ezh1 regulates expression of Cpg15/Neuritin in mouse cortical neurons.; Authors - Shun Utsunomiya ; KO Validated
human	WB	Photochem Photobiol; The m6 A reader YTHDC2 regulates UVB-induced DNA damage repair and histone modification; Authors - Zizhao Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mengxue (Verified Customer) (11-29-2018)

This is the 5th EZH1 Ab I've tried so far and the only one that works with our rat primary cells. I got clear band on Western and I'm so happy about it!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat primary fibroblast cells

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