Anticorps Polyclonal de lapin anti-EZH2

• Phare
• Validé par KD/KO

EZH2 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

74

Avis (1)

N° de cat : 21800-1-AP

Synonymes

Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

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Galerie de données de validation

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  WB analysis using 21800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 21800-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21800-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 21800-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21800-1-AP

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21800-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21800-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21800-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21800-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21800-1-AP

  IP Result of anti-EZH2 (IP:21800-1-AP, 4ug; Detection:21800-1-AP 1:1000) with HepG2 cells lysate 3400ug.

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  IF Staining of HepG2 using 21800-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21800-1-AP (EZH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 21800-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (21800-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules A431, cellules C6, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules Raji, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 18 publications below
• WB: See 64 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 6 publications below
• RIP: See 1 publications below

Informations sur le produit

21800-1-AP cible EZH2 dans les applications de WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EZH2 Protéine recombinante Ag16789
• Nom complet: enhancer of zeste homolog 2 (Drosophila)
• Masse moléculaire calculée: 751 aa, 86 kDa
• Poids moléculaire observé: 90-102 kDa
• Numéro d’acquisition GenBank: BC010858
• Symbole du gène: EZH2
• Identification du gène (NCBI): 2146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Publications

Species	Application	Title
human	WB	Circ Res; VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression.; Authors - Marco F Morini
human	WB,IF	Nat Struct Mol Biol; lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal.; Authors - Pingping Zhu
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li
human,mouse	WB	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li ; KD Validated
human	WB	Nat Commun; CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.; Authors - Yongqing Liu ; KD Validated
human	WB	Clin Cancer Res; Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.; Authors - Huanjun Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikhil (Verified Customer) (10-16-2019)

very nice

• Applications: Western Blot,
• Cell Tissue Type: Colon cancer cells

EZH2 Antibody Western Blot, validation ( dilution) in Colon cancer cells (Cat no:21800-1-AP)

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