Anticorps Polyclonal de lapin anti-Ezrin

• Phare
• Validé par KD/KO

Ezrin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 26056-1-AP

Synonymes

EZR, VIL2, Villin-2

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Galerie de données de validation

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  WB analysis of HeLa using 26056-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26056-1-AP

  WB result of Ezrin antibody (26056-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ezrin transfected HeLa cells.

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  IP experiment of HeLa using 26056-1-AP

  IP result of anti-Ezrin (IP:26056-1-AP, 4ug; Detection:26056-1-AP 1:2000) with HeLa cells lysate 3200ug.

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:8000 (under 40x lens).

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  IHC staining of human small intestine using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human small intestine using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens).

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  IF Staining of mouse eye using 26056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Ezrin antibody (26056-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 26056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Ezrin antibody (26056-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat eye using 26056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Ezrin antibody (26056-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26056-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ezrin antibody (26056-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  FC experiment of HeLa using 26056-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Ezrin Polyclonal antibody (26056-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'estomac de souris, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de rat, tissu oculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

26056-1-AP cible Ezrin dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ezrin Protéine recombinante Ag23530
• Nom complet: ezrin
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC013903
• Symbole du gène: Ezrin
• Identification du gène (NCBI): 7430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.

Publications

Species	Application	Title
mouse,human	IF	Acta Neuropathol; Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.; Authors - Matthew J Broadhead
human	WB	Proc Natl Acad Sci U S A; Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.; Authors - Krishnendu Khan ; KO Validated
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan ; KD Validated
mouse	WB	J Biol Chem; Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.; Authors - Songhua Li
pig	WB,IF	J Virol; Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin; Authors - Shujuan Zhang

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