Anticorps Polyclonal de lapin anti-PAR1/Thrombin Receptor

• Phare
• Validé par KD/KO

PAR1/Thrombin Receptor Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 26366-1-AP

Synonymes

F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1

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Galerie de données de validation

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  WB analysis using 26366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26366-1-AP (PAR1/Thrombin Receptor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26366-1-AP

  WB result of F2R antibody (26366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAR1/Thrombin Receptor transfected K-562 cells.

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  IHC staining of human liver using 26366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules K-562
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

26366-1-AP cible PAR1/Thrombin Receptor dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAR1/Thrombin Receptor Protéine recombinante Ag24378
• Nom complet: coagulation factor II (thrombin) receptor
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC051909
• Symbole du gène: F2R
• Identification du gène (NCBI): 2149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Breast Cancer Res Treat; Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.; Authors - Yihui Zhu
hamster	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu
human,monkey	WB,IHC,IF	Genes Immun; A Golgi apparatus-related signature predicts the immune microenvironment and prognosis of gastric cancer; Authors - Changlei Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (02-15-2024)

Several unspecific bands detected by WB one of which was the correct size. IF staining worked well but caveated by potential lack of specificity.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 1000 WB 1 in 200 IF
• Cell Tissue Type: HUVEC lysate

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