Anticorps Polyclonal de lapin anti-PAR2

• Phare
• Validé par KD/KO

PAR2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12160-1-AP

Synonymes

F2RL1, G protein coupled receptor 11, GPR11, PAR 2, PAR2, Thrombin receptor like 1

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Galerie de données de validation

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  WB analysis of COLO 320 using 12160-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12160-1-AP (PAR2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules COLO 320,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below

Informations sur le produit

12160-1-AP cible PAR2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAR2 Protéine recombinante Ag2801
• Nom complet: coagulation factor II (thrombin) receptor-like 1
• Masse moléculaire calculée: 397 aa, 44 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC018130
• Symbole du gène: F2RL1
• Identification du gène (NCBI): 2150
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteinase-activated receptors (PARs) are G protein-coupled receptors activated through cleavage of their N-termini by mainly serine proteases. The family of PARs includes four members: PAR1, PAR2, PAR3, and PAR4. PAR2, also known as F2RL1, is expressed in epithelial cells (e.g., lung, gastrointestinal tract), endothelial cells, smooth muscle cells, fibroblasts, nerves, and some immune and inflammatory cells. PAR2 knockout mice and PAR2 agonists and antagonists have implicated PAR2 as a promising target in inflammatory conditions; respiratory, gastrointestinal, metabolic, cardiovascular, and neurological dysfunction; and cancers. (PMID:23895492)

Publications

Species	Application	Title
		J Med Chem; Toward drugs for protease-activated receptor 2 (PAR2).; Authors - Mei-Kwan Yau
human	WB	Cells; Modulation of Intestinal Epithelial Permeability via Protease-Activated Receptor-2-Induced Autophagy.; Authors - Yuju Kim
human	WB	Biochem J; Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.; Authors - Chunyang Du
human	WB	Med Oncol; Long non-coding RNA AC245100.4 contributes to prostate cancer migration via regulating PAR2 and activating p38-MAPK pathway.; Authors - Chi Liu ; KD Validated
human	WB	Heliyon; Long non-coding RNA AC245100.4 activates the PI3K/AKT pathway to promote PCa cell proliferation by elevating PAR2; Authors - Ke Zhang
human,rat	WB	Mediators Inflamm; Protease-Activated Receptor-2 and Phospholipid Metabolism Analysis in Hyperuricemia-Induced Renal Injury; Authors - Xiaolu Sui ; KD Validated