Anticorps Polyclonal de lapin anti-F4/80

F4/80 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(308)

Avis (1)

N° de cat : 28463-1-AP

Synonymes

Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, Emr1

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Galerie de données de validation

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  WB analysis using 28463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28463-1-AP (F4/80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of RAW 264.7 using 28463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (28463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: unboiled J774A.1 cells, unboiled RAW 264.7 cells
• Résultats positifs en IHC: tissu splénique de souris, tissu cardiaque de souris, tissu cérébral de souris, tissu d'intestin grêle de souris, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris, tissu hépatique de souris
• Résultats positifs en IF-Fro: tissu splénique de souris,
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 147 publications below
• IF: See 139 publications below

Informations sur le produit

28463-1-AP cible F4/80 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: F4/80 Protéine recombinante Ag28252
• Nom complet: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 130 kDa, 160 kDa
• Numéro d’acquisition GenBank: NM_010130
• Symbole du gène: F4/80
• Identification du gène (NCBI): 13733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publications

Species	Application	Title
	IF	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang
mouse	IF	Nat Commun; Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway; Authors - Yanan Zhang
human	IF	Nat Commun; Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure; Authors - Rui Wang
rat,human	WB	Adv Sci (Weinh); Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury; Authors - Qiaohui Chen
	IF	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
human	IF	Cell Rep Med; Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma; Authors - Junju Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kannan (Verified Customer) (06-13-2023)

AR using EDTApH9. overnight incubation of primary antibody at 4c.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse uterus

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