Anticorps Polyclonal de lapin anti-F4/80

F4/80 Polyclonal Antibody for IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

N° de cat : CL488-28463

Synonymes

F4 80, Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1

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Galerie de données de validation

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  IF Staining of mouse spleen using CL488-28463

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 F4/80 antibody (CL488-28463) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using CL488-28463

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 F4/80 antibody (CL488-28463) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using CL488-28463

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 F4/80 antibody (CL488-28463) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu splénique de souris,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-28463 cible F4/80 dans les applications de IF-P et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: F4/80 Protéine recombinante Ag28252
• Nom complet: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Masse moléculaire calculée: 102 kDa
• Numéro d’acquisition GenBank: NM_010130
• Symbole du gène: F4/80
• Identification du gène (NCBI): 13733
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publications

Species	Application	Title
	IF	Neural Regen Res; Changes in border-associated macrophages after stroke: single-cell sequencing analysis; Authors - Ning Yu

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