Anticorps Polyclonal de lapin anti-Factor VII

Factor VII Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 23058-1-AP

Synonymes

F7, Coagulation factor VII, EC:3.4.21.21, Eptacog alfa, Proconvertin

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Galerie de données de validation

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  WB analysis using 23058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 23058-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23058-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 23058-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 23058-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using F7 antibody (23058-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, plasma humain, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

23058-1-AP cible Factor VII dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Factor VII Protéine recombinante Ag19324
• Nom complet: coagulation factor VII (serum prothrombin conversion accelerator)
• Masse moléculaire calculée: 466 aa, 52 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC130468
• Symbole du gène: F7
• Identification du gène (NCBI): 2155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
mouse	WB	Drug Des Devel Ther; Guanxinning Tablet Alleviates Post-Ischemic Stroke Injury Via Regulating Complement and Coagulation Cascades Pathway and Inflammatory Network Mobilization; Authors - Yule Wang
pig	WB	Vet Res; TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus; Authors - Xuejiao Zhu ; KD Validated

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