Anticorps Polyclonal de lapin anti-FABP2

• Phare
• Validé par KD/KO

FABP2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 21252-1-AP

Synonymes

Intestinal-type fatty acid-binding protein, I-FABP, Fatty acid-binding protein, intestinal, Fatty acid-binding protein 2, Fatty acid binding protein 2

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Galerie de données de validation

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  WB analysis of COLO 320 using 21252-1-AP

  WB result of FABP2 antibody (21252-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells.

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  WB analysis using 21252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21252-1-AP (FABP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21252-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21252-1-AP (FABP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21252-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21252-1-AP (FABP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21252-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21252-1-AP (FABP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below

Informations sur le produit

21252-1-AP cible FABP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP2 Protéine recombinante Ag15793
• Nom complet: fatty acid binding protein 2, intestinal
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC069617
• Symbole du gène: FABP2
• Identification du gène (NCBI): 2169
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP2, also known as Intestinal Fatty Acid Binding Protein (I-FABP), is a member of the Fatty Acid Binding Proteins (FABPs) family. The cytoplasmic content of FABP2 could be delivered into the systematic circulation once the death of enterocyte happens, thus the elevated FABP2 concentration in the blood has been shown in many human intestinal diseases. FABP2 is a water soluble cytosolic protein with a small molecular weight of 14-15 kDa, and it is initially located in the mature enterocytes of the small intestine (PMID: 26547205).

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	Cell Mol Gastroenterol Hepatol; FACI Is a Novel CREB-H-Induced Protein That Inhibits Intestinal Lipid Absorption and Reverses Diet-Induced Obesity.; Authors - Yun Cheng
rat	WB	Int J Mol Sci; INT-767-A Dual Farnesoid-X Receptor (FXR) and Takeda G Protein-Coupled Receptor-5 (TGR5) Agonist Improves Survival in Rats and Attenuates Intestinal Ischemia Reperfusion Injury; Authors - Emilio Canovai
mouse	WB	Microb Biotechnol; Faecal microbiota transplantation-mediated jejunal microbiota changes halt high-fat diet-induced obesity in mice via retarding intestinal fat absorption; Authors - Luoyi Zhu
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo

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